Curcuma longa debranched starch assisted synthesis of cerium oxide nanoparticles and its antioxidant, anticancer, antimicrobial, and anti-biofilm activities
来源：X-MOL
Nowadays, there is a growing emphasis on eco-friendly methods for synthesizing metal nanoparticles (NPs), that avoid chemical processes and the production of harmful substances. In this study, we present a novel green approach for preparing cerium oxide nanodots (CeO2NPs) using Curcuma longa debranched starch as a reducing and capping agent via the sol-gel method. The prepared CeO2NPs were characterized using various techniques, including UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and zeta potential (ζ). An absorption peak of the particles at 315 nm was detected in the UV-Vis spectrum. Spherical CeO2NPs were observed by SEM and TEM with a 2–4 nm NP range. Energy-dispersive X-ray spectroscopy (EDX) analysis showed that the Ce, oxygen and carbon contents were 68.33 wt%, 25.59 wt and 6.07 wt%, respectively. The CeO2NPs exhibited antioxidant activity with an IC50 value of 3.2 ± 0.23 µg/mL, and 3.66 ± 0.18 µg/mL for DPPH and ABTS free radical scavenging assays, respectively. Furthermore, CeO2NPs exhibited antibacterial activity against Corynebacterium diphtheriae, Klebsiella pneumoniae, Salmonella typhi, and Escherichia coli and antibiofilm activity against Corynebacterium diphtheriae and Escherichia coli. The in vitro cytotoxicity of CeO2NPs against HepG2 cells (IC50 of 178 ± 14 µg/mL) was dose-dependent as evaluated using the MTT assay. These results collectively suggest the diverse functions of CeO2NPs, showcasing their potential as antioxidants, anticancer and antibacterial agents, and their usefulness in biomedical applications.